Research Article CODEN: IJPNL6 A SENSITIVE DUAL-RUN HPTLC TECHNIQUE FOR SIMULTANEOUS ESTIMATION OF METFORMIN HYDROCHLORIDE, GLIMEPIRIDE AND VOGLIBOSE IN COMBINED DOSAGE FORM

A sensitive, selective, and precise high performance thin layer chromatography (HPTLC) based on dual-run technique has been developed for the simultaneous estimation of metformin (MET), glimepiride (GLI), and voglibose (VOG) in combined dosage form. Chromatographic separation was performed on aluminium plates precoated with silica gel 60 F254 as the stationary phase. A dual run technique was adopted for better resolution amongst all three drugs with solvent system initially toluene-methanol-ethyl acetate-formic acid (3:4:3:0.5, v/v/v/v) for separation of metformin. The plates were dried and developed again using another mobile phase system consisting of toluene-ethyl acetate-formic acid (5:5:1, v/v/v) for glimepiride and voglibose. Densitometric scanning was carried out in the absorbance mode at 254 nm. The linear regression data for calibration plots showed good linear relationship in the concentration range of 200-1200 ng/band for MET, 20-120 ng/band for GLI and VOG. The method was validated for precision, accuracy, robustness and recovery as per ICH guidelines and applied for quantification in their available combined pharmaceutical dosage form. Consider the merits of dual-run mode and sensitivity, the proposed method would be novel for the determination of MET, GLI and VOG