International Journal of Pharma and Bio Sciences PHYTOCHEMICAL ANALYSIS, IN VITRO ANTI-BACTERIAL AND ANTIOXIDANT ACTIVITIES OF WILD ONION SPS.

The white bulbs of ‘Wild Onion, Urginea indica ’ samples were collected from the hillocks near Vaddagere village of Tumkur district, a southern part of Karnataka. The study was performed extensively for the first time, leading to the evaluation of active constituents from wild onion bulb. The extract was prepared with Methanol and subjected for preliminary phyto-chemical and physico-chemical analysis. The total ash content, acid insoluble and water soluble ash content were evaluated along with the fluorescence characteristics of the methanolic extract of Wild onion sps. The presence of primary and secondary metabolites such as carbohydrate, proteins, alkaloids, phenolic compounds, saponins was confirmed through preliminary phyto-chemical analysis. The extract was found to possess anti-bacterial activity in E. coli, S. aureus and P. aeruginosa isolated from infected patients. The Minimum inhibitory concentration (MIC) was also evaluated by ‘Tube dilution ’ method and the result was found to be considerably effective against selected pathogenic bacteria. Such an effect might contribute to explaining the traditional use of wild onion sps, Urginea indica in the treatment of wound healing. The antioxidant activity was estimated by using DPPH free radical scavenging assay and the activity was increased with increase in concentration of methanolic fraction of wild Onion sps