www.ijstr.org Effect of Locally Applied Black-seed Oil and Honey Mixture on Wound Healing

Abstract: Back ground: Wound healing is a complex process, which is influenced by multiple factors. Wound management still remains an important focus of researches. The use of natural products as an alternative treatment has been on the rise in the last few decades. blackseed and honey are of the oldest alternative medicine. Objective: The aim of this study is to evaluate the effect of locally applied blackseed oil and honey mixture on experimental wound healing in rabbit. Material and methods: Twenty four young male rabbits from animal house, College of Medicine, University of Mosul were used in this study at 1st of June to 30 of November 2012. The animals divided into two groups after induction of circular wound by 4 mm sharp circular punch in both ears of rabbit under anesthesia. The first group is experimental, the wounds treated daily by local application of black seed oil and honey mixture, the second group is control, no local treatment applied. In the end of 1st, 2nd, 3d, 4th weeks three animals from both group were anesthetized and the wound area and excised. The wound area estimated and the specimens prepared for histological examination for the wounds healing. Results: The mean wound areas in experimental group at the end of 1st, 2nd, 3rd, 4th were 22, 11.7, 7. 5, and 2.3 respectively, while in control group were 22.3, 13.8, 10.5, and 7 respectively, the difference between two means was significant only at end of 4th week ( p value = 0.003). The histological examination of wound site in experimental group at 1st, 2nd, 3rd, and 4th weeks shows histological scoring 6.12, 8.33, 10. 66, and 11.5 respectively, while in control group 5.66, 7.56, 8.2, and 10.16 respectively. The difference between the two means was significant at the end of 3rd and 4th week. Conclusion: The present study demonstrates that a locally applied blackseed oil and honey mixture enhances healing in experimental wound in rabbit ear