RESEARCH Induction of robust immu by dual-expression-system

Full list of author information is available at the end of the articleBackground Porcine circovirus type 2 (PCV2), a member of the family Circoviridae, is recognized as the causative agent of the various clinical manifestations of PCV-associated diseases (PCVAD), although other factors influence the severity of the clinical symptoms [1]. To combat the growing problems associated with PCV2-associated disease, several commer-cial PCV2 vaccines have been developed and been shown to provide some degree of protection against PCV2 infection [2,3]. In the present study, a new approach to the prevention and control PCV2 was explored using a baculovirus dual expression system to express the capsid (Cap) protein of PCV2 as a subunit vaccine and a DNA vaccine. The baculovirus Autographa californica multiple nu-clear polyhedrosis virus (AcMNPV) has been used trad-itionally as an excellent tool to overexpress recombinant proteins in insect cells [4-6]. Because the baculoviru