Abstract: Background: Lack of proper knowledge of

baseline titre of Widal test can lead to over di-agnosis of typhoid fever leading to mismanage-ment of patients. Rapid semiquantitative slide Widal test has replaced conventional tube Widal test in many laboratories, but there is paucity of scientific data regarding how far the results of this rapid test correlate with that of conven-tional tube Widal test. Aims and Objectives: To evaluate baseline titre of widal using quanti-tative tube Widal test, to suggest the cut-off value for positive Widal test in this region, and to correlate the results obtained in quantitative tube Widal test with that of rapid semiquantitative slide Widal test. Material and Methods: Sera of 107 apparently healthy blood donors (AHDs) were subjected to quantitative tube and semiquantitative slide Widal test to know the titre. Results: Highest titre obtained by tube Widal test for TO was 1:160, for TH-1:320, for AH- 1:20, and for BH- 1:80. Tube Widal titres of < 1:80 for TO and TH were see