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Partial characterization and optimization of protease production from newly isolated Cohnella thermotolerans from Lonar Lake Lonar Lake, an impact crater located in the Buldhana district of Maharashtra State, India is occupied by saline water and harbors various unidentified, unique haloalkaliphilic bacterial bacillus species which produces thermo-halo-alkaliphilic proteases. The present study deals with the isolation, production dynamics, purification, characterization and optimization of a protease from Cohnella thermotolerans isolated and identified by 16s RNA ribotyping from the Alkaline Lonar Lake. The C. thermotolerans produced protease at maximum rate after 72 h of incubation at 370C with the agitation speed of 120 rpm and 5 % of starter culture. The best carbon sources for this C. thermotolerans were fructose and maltose and best nitrogen source was yeast extract, while the most effective inorganic nitrogen source was ammonium carbonate. Supplementation of the culture medium with amino acid L-glutamic acid and metal ion Mg2+ improved the protease production substantially. Under these conditions, newly isolated Cohnella thermotolerans strain was found to produce alkaline protease at a maximum rate of optimum pH 10 and temperature a