Alterations of Rat Intestinal Disaccharide Absorption by Bile or Conjugated Bile Acids

Summary: Taurocholate showed different effects on the jejunal sucrose absorption, depending on the concentration of the bile acid: 3 mmol/1 taurocholate inhibited to a small extent (- 7%), 10 mmol/1 taurocholate had no effect and 30 mmol/1 taurocholate increased the sucrose absorption by 17%. The sucrose hydrolysis was augmented by 20 % in the presence of 30 mmol/1 taurocholate. 2 mmol/1 taurochenodeoxycholate caused an increase of sucrose hydrolysis (+ 37%) as well as of sucrose absorption (+12%) in the jejunum. Pooled rat bile induced a 21 % inhibition of the sucrose hydrolysis, while the sucrose absorption was unchanged. The jejunal maltose hydrolysis was un-affected by 3 mmol/1 taurocholate, whereas 10 or 30 mmol/1 caused a minimal increase (+3%). The absorption of the resulting glucose was reduced to 84-92%. In the ileum 10 mmol/1 taurocholate did not change the sucrose hydrolysis and absorption, whereas 2 mmol/1 taurochenodeoxycholate induced a 30 % decrease. The investigated bile acids had no effect on the microscopic structure of the small intestinal mucosa. Hypertonie solutions caused an accumulation and exfoliation of PAS-positive material at the top of the villi, independent of the presence or absence of bile acids. Änderungen der intestinalen Disaccharidabsorption der Ratte durch Galle oder konjugierte Gallensäuren Zusammenfassung: Taurocholat hatte —konzentrationsabhängig — unterschiedliche Effekte auf die Saccharoseabsorp