Spinal Fluid Protein Revisited: A Reappraisal of the Biuret Procedure

Summary: In this study our previously described selected method, a biuret procedure with deproteinization, for the determination of spinal fluid protein is thoroughly discussed against the background of the results found with a number of Lowry modifications. The use of various Separation techniques, i.e. deproteinization, Ultrafiltration and chromatography (HPLC), for protein analysis ledj;o the question äs to whether low molecular weight proteins in cerebrospinal fluid play an important role or not with respect to the choice of a selected method for the determination of total protein. Erneute kritische Überprüfung der Eignung des Biuretverfahrens zur Bestimmung des Liquorproteins Zusammenfassung: Unsere kürzlich für die Bestimmung von Liquorprotein beschriebene ausgewählte Me