The Influence of Bilirubin, Haemolysis and Turbidity on 20 Analytical Tests Performed on Automatic Analysers Results of an interlaboratory study

Summary: The director of a laboratory has to be sure to give out reliable results for routine tests on automatic analysers regardless of the clinical context. However, he may find hyperbilirubinaemia in some circumstances, parenteral nutrition causing turbidity in others, and haemolysis occurring if sampling is difficult. For this reason, the Commission for Instrumentation of the Societe Frangaise de Biologie Clinique (SFBC) (president Alain Feuillu) decided to look into "visible " interferences — bilirubin, haemolysis and turbidity — and their effect on 20 major tests: 13 substrates/chemistries: albumin, calcium, cholesterol, creatinine, glucose, iron, magnesium, phosphorus, total bilirubin, total proteins, triacylglycerols, uric acid, urea, and 7 enzymatic activities: alkaline phosphatase, alanine aminotransferase,-amylase, aspartate aminotransferase, creatine kinase,-glutamyl transferase and lactate dehydrogenase measured on 15 automatic analysers representative of those found on the French market (Astra 8