Variations in Human Serum Lipoprotein Detected by Isoelectric Focusing

Following the method of KOSTNER and coworkers with some modifications, human serum lipoproteins of native serum and isolated fractions were studied by means of isoelectric focusing with ampholine 3—10 (LKB Instruments). Lipoprotein fractions were prepared by sequential preparative ultracentrifugation at increasing densities. Sera and fractions from 10 healthy donors were repeatedly investi-gated to evaluate possible environmental factors on the expression of banding patterns. As a result, at least two of the observed bands seem to vary depending on whether the sera were obtained from fasting or nonfasting individuals. The investigation of 158 related in-dividuals yielded some insight into a possible genetic determination of specific bands. It may however be difficult to distinguish all genetic and environmental factors involved. Isoelectric focusing may soon be of great clinical value in the diagnosis of type I to V hyperlipo-proteinemia. In Anlehnung an die Methode von KOSTNER und Mitarbeitern wurden menschliche Serumlipoproteine in Vollserum und isolierten Frak