Comparison of the Reflectance Method (Reflotron ® Reflectance Photometer) with the Absorbance Method (Automatic Analysers) for the Determination of Cholesterol

Education Program (2) have issued detailed guide values for recognition and management of hyperlipidaemia in adults. In these guidelines, the diagnosis of dyslipidaemia based on the measurements of total Cholesterol, triacylglycerols, HDL and LDL Cholesterol plays an important role. A prerequisite for the desired success of interventive measures is the reliability of the analytical data. The aim of this study was to investigate the precision and accuracy of Reflotron ® Cholesterol, a method based on the dry chemistry principle. Accuracy was assessed by establishing the correlation with the standardized automated methods used in routine lipid diagnosis. In addition, it was also examined whether the Reflotron ® Cholesterol results in plasma and blood are comparable. The Reflotron ® Cholesterol (sample: blood) showed a good correlation with the CHOD/PAP method on a Hitachi 737 Instrument (sample: plasma). The median value of the differences of the test results was-^0.4%. Similarly, the method comparison of Reflotron ® Cholesterol (sample: blood) versus CHOD/PAP method on a SMAC Instrument (sample: plasma) showed that Reflotron ® produces slightly (1.8%) higher results. The Refloton ® Cholesterol values obtained from blood samples were slightly lower thän those ffom plasma samples (median value of the differences: —2.2%). The results $iiggest thät for routine purposes Reflotron ® Cholesterol provides results which are in good agreement with those obtained by standardized wet chemistry methods