Quantitative Differentiation of Human Serum Alkaline Phosphatase Isoenzymes with Polyacrylamide Disc Gel Electrophoresis

The separation of alkaline phosphatase (EC 3.1.3.1) isoenzymes by means of polyacrylamidc disc gel electrophoresis is described. The electrophoretic quantitation of the alkaline phosphatase isoenzymes and the ratio of bone-type fraction/liver-type fraction gives more clinical information than the heat-inactivation method. It is demonstrated that the described electrophoretic method together with the determination of y-glutamyl-transpeptidase (EC 2.3.2.1) and S'-nucleotidase (EC 3.1.3.5) is a reliable and a satisfactory method in the routine hospital laboratory for the differentiation of bone and hepatobiliary diseases. Die Trennung der Isoenzyme der alkalischen Phosphatase (EC 3.1.3.1) mit der Polyacrylamidgel-Disc-Elektrophorese wird beschrieben. Die elektrophoretische Bestimmung der Isoenzyme der alkalischen Phosphatase und des Quotienten Knochentyp-Fraktion/Lebertyp-Fraktion gibt mehr Information für die Klinik als die Hitze-Inaktivierungs-Methode