A New Method for the Determination of Nucleotidase in Serum Automated determination of the 5'-nucleotidase by continuous flow analysis without dialysis

A fully automated procedure is proposed for the direct determination of 5'-nucleotidase in serum, using only a simple manifold in a continuous flow technique without dialysis. Ein automatisches Verfahren nach dem Prinzip der kontinuierlichen Durchflußanalyse für die Bestimmung von 5'-Nucleotidase im Serum wird beschrieben. Es wird ein einfaches Fließ diagram ohne Dialyse benutzt. In pfevious reports (1—6) we described a new technique for the manual determination of serum S'-nucleotidase1). This report presents a simple automated adaption to continuous flow analysis of the above mentioned manual procedure. We optimalised the flow scheme making use of the mathematical model described by Walker et al (7). For the determination of the most favourable sample rate (sample time and sample/wash ratio) the curve characteristics "lag phase " and "experimental factor" seem to be of the greatest importance. They can b