Unconjugated 5a-Androstan-3a,17j3-diol and 5a-Androstane-30,17ß-diol in Human Plasma as Measured by Radioimmunoassay without Chromatography

Summary: A radioimmunoassay (RIA) without chromatography for the determination of 5a-androstan-3a,17j3-diol and of 5a-androstane-3(3,17/3-diol in human plasma by using highly specific antisera against the 15/3-carboxyethyl-mercapto-bovine serum albumin conjugates is described. It could be shown that the 13 % cross reaction of the 5a-androstane-3/3,17/3-diol antiserum with pure androst-5-ene-3/3,17/3-diol was negligibly low in plasma extracts. Sensivity, accuracy, precision, and linearity revealed results which comply with the requisites of a reliable RIA. The plasma levels obtained were as follows (mean ± SD): 5a-androstan-3a, 17/3-diol: Normal males (N = 27) 0.86 ± 0.22 nmol/1; normal females (N = 10) 0.31 ± 0.07 nmol/1; hirsute females (N = 25) 0.60 ± 0.24 nmol/1; 5a-androstane-3/3,170-diol: Normal males 1.47 ± 0.43 nmol/1; normal females 0.49 ±0.10 nmol/1; hirsute females 0.91 ± 0.32 nmol/1. Unkonjugiertes 5a-Androstan-3a, l Jfrdiol und 5a-Androstan-3ß, 17ß-diol im Plasma des Menschen, mit Radioimmunoassay ohne Chromatographie bestimm