Lipid Screening by Thin Layer Chroniatography

A thin layer Chromatographie method for the analysis of lipids in serum is described. Sterol esters, triglycerides, free sterols, free fatty acids, and phospholipids can be separated. After charring by heating with 10 % sulphuric acid and clearing with a clearing solution, the thin layer chromatograms can be scanned with the Analytrol Scanner. This method permits phenotyping of hyperlipoproteinaemia, and a rapid evaluation of the ratio of the five most important lipid components can be made. Eine dünnschichtchromatographische Methode zur Bestimmung der Serumlipide wird beschrieben. Sterinester, Triglyceride, freie