Cholesterol in High-Density Lipoproteins: A Comparison Between Dextran Sulfate-Magnesium Chloride Precipitation and Preparative Ultracentrifugation

Summary: The cholesterol in high density lipoproteins (HDL) has been determined in 140 serum samples after dex-tran sulfate-magnesium chloride precipitation of the apolipoprotein B-containing lipoproteins. The values correlated well with simultaneously measured values from preparative ultracentrifugation (slope 0.98, r = 0.93). Furthermore, there was a significant correlation in 60 specimen between the dextran sulfate-MgC!2 and heparin-MnCl2-precipitation method. Dextran sulfate-MgQ2 precipitation allows a precise and reproducible HDL-cholesterol determination (CV from 20 days 2.7 %). Cholesterin in Lipoproteinen hoher Dichte: Ein Vergleich zwischen Dextransulfat-Magnesiumchloridpräzipitation und präparativer Ultrazentrifugatio