Adamski and Kochana: Polypyrrole Layers Electrodeposited from TRIS Buffer... Short communication Polypyrrole Layers Electrodeposited from TRIS Buffer Solution as Matrix of Tyrosinase Biosensor

A novel supporting electrolyte – tris(hydroxymethyl)aminomethane hydrochloride (TRIS) buffer solution was used to obtain polypyrrole (PPy) layers based on the anodic oxidation of pyrrole. Influence of some parameters: temperature, monomer and supporting electrolyte concentration, polarization potential and hydrodynamic conditions on electrodepo-sition process were investigated. Obtained layers were preliminary tested in construction of an amperometric biosensor based on enzyme tyrosinase, which response was tested using catechol as a substrate