Developmental Biology of wood formation- Finding regulatory factors through functional genomics

The wood-forming vascular cambium is responsible for the production of a large part of the biomass on this planet. Yet, there is only limited knowledge on how cell proliferation and differentiation in the cambial meristem are regulated. In this thesis the wood-forming tissues of aspen were used as a model system to identify and characterize molecular factors related to cambial meristem activity. An important regulator of cambial meristem activity is the plant hormone auxin. As polar transport is crucial for the delivery of auxin to the cambial zone, we identified homologues of known regulators of polar auxin transport and described their regulation by environmental and developmental factors. Translating changes in auxin concentration into changes in gene expression involves members of the Aux/IAA gene family. Aspen homologues of Aux/IAA genes were cloned and found to be expressed in a highly tissue-specific fashion, which is further influenced by developmental events and changes in the environment. A major response of trees to environmental changes is the suspension of meristematic growth during winter dormancy. A comparison of gene expression in active and dormant cambia revealed dramatic changes in the transcriptome including the expression of many col