ULTRASTRUCTURE OF CERVICAL INTRAEPITHELIAL NEOPLASIA TYPE 3 (CIN3) WITH HUMAN PAPILLOMAVIRUS (HVP) INFECTION.

The cervical intraepithelial neoplasia (CIN) is a type of precursor lesion to squamous cell carcinoma of cervix. Frequently these lesions show cytological changes suggestive of HPV infection . These viruses can infect the anogenital tract; some cutaneous HPVs such as type 2 can infect mucosa epithelium and HPV 16, which is found in association with invasive squamous cell carcinomas . Based on their associations with specific types of lesion, the high oncopenic risk HPVs are type 16, 18, 45 and 56. Methods: Cervical biopsies were obtained of thirteen patients with diagnosis of CIN 3 admited to the consult of cervix pathology Maria Teresa Toro at Aragua, Venezuela. The viral DNA was detected by polymerase chain reaction (PCR) and biopsies were processed with routine techiques for transmission electron microscopy. Results: HPV DNA was detected IN 30,7 % of CIN 3, in 38,4 % it wasn’t and 30,7 % was insufficient matter. The CIN 3 positive HPV DNA showed nuclear enlargement, nuclear hipercromaticity and capillaries with lysosome. The CIN 3 negative HVP DNA showed pynocitotic vesicles, abnormal mitochondria, lipofuscin granules, capillaries with macrophages, myelin-like figures, all this has been reported in reparative processes