A comparison of methods for retrieval of DNA and RNA from FFPE tissues

A variety of commercially available kits designed for the purpose of extracting DNA/RNA from formalin-fixed, paraffin-embedded (FFPE) samples were tested under controlled conditions. The kits were selected based on the method of extraction: Column-based, lysis only, solid-phase reverse binding, and magnetic based extraction. Kits were compared on the basis of resulting nucleic acid concentration, yield, protein and chemical contamination, and maximum amplifiable fragment length. The results of this study indicate that each type of kit has distinct advantages and a careful consideration should be given to ultimate experimental requirements prior to purchasing the kit