In vitro Antimicrobial Activity of the Volatile Oil of

The in-vitro antimicrobial activity of the Volatile oils of Nigella Sativa Linn Seeds was tested against fifteen pathogenic microbial strains includung three gram-positive, eleven gram-negative and a yeast Candida albicans. The volatile oil showed strong sensitivity to all the organisms. The zone of inhibition was found 13-32mm at a dose of 600µg/disc. Minimum inhibitory concentration (MIC) of the volatile oil was also determined against Staph. Aureus ATCC and E.coli ATCC were found 187µg/ml and 375µg/ml respectively