Wheat streak mosaic virus cylindrical inclusion body protein

A protein of apparent molecular weight 66000 was purified from wheat plants infected with wheat streak mosaic virus. Ant iserum to this protein, labelled with gold, specifically stained cylindrical inclusions in ultrathin sections of virus-infected cells. Ant iserum to the Mr 66000 protein did not react with capsid protein in Western blots, nor did antiserum to capsid protein react with the Mr 66000 protein. Both antisera reacted with homologous antigens. The concentrat ion of the Mr 66000 protein in extracts from infected leaves was about 100 ~g per g of leaves, which is higher than the usual concentrat ion of virions