DOI:10.2298/JSC091207086Z 1 Chelate-assisted phytoextraction: effect of EDTA and EDDS on copper uptake by Brassica napus L.

Abstract: Chelate-assisted phytoextraction has been proposed as an effective approach to removing heavy metals from contaminated soil through use of high biomass plants. The aim of the present study was to compare the efficiency of the two chelators: EDTA and biodegradable EDDS in enhancing Cu uptake and translocation by Brassica napus L. grown on moderately contaminated soil and treated with increasing concentrations of EDTA or EDDS. Increasing amounts of EDDS caused a serious growth depression of Brassica napus and an increase in shoot metal concentrations. Growth depression limited the actual amount of phytoextracted Cu at high concentrations of EDDS. The maximum amount of extracted Cu was achieved by the application of 8.0 and 4.0+4.0 mmol/kg EDDS. The shoot Cu concentrations after EDTA application were much lower than with EDDS at the same doses. According to this experiment, EDTA does not appear to be an efficient amendment if Cu phytoextraction with Brassica napus is considered but EDDS is