NAD Metabolism in Sdmmllu typhimurium: Isolation of Pyridine Analogue Supersensitive (p) andp Suppressor Mutants

Mutants of Salmonello typhinturiwrt supersensitive to the nicotinic acid analogue &amino-nicotinic acid (6ANA) were isolated as unable to grow on what are normally subinhibitory concentrations of the analogue. The mutations were classified on the basis of their map positions aspasA (89-92 units),psB ( W 9 units),pasC(18-22 units),pasD (18 units) andpasE(55 units). The mutants exhibited a wide range of minimal inhibitory concentrations towards 6ANA, and several were affected in terms of growth. The data suggest that most of the mutations probably reside in genes whose products utilize nicotinamide adenine dinucleotide (NAD) as a cofactor, the altered gene products being more sensitive to internal Gamin0 NAD concentrations. Secondary mutations which suppress the Pas- phenotype were found to reside in the following NAD-related loci; pncB, mdB and nudD. Two of the pncB mutants appear to be affected in the expression of NAPRTase while several of the mzdB mutants are apparently insensitive to feedback inhibition by internal NAD concentrations