Determination of isotretinoin in pharmaceutical formulations by reversed-phase HPLC

The development of facile and rapid quantification of biologically active biomolecules such as isotretitoin in therapeutic drugs contained in many generic formu-lations is necessary for determining their efficiency and their quality to improve the human health care. Isotretritoin finds its applications in the maintenance of epithelial tissues. Different processes to date such as normal phase HPLC, or gas chromatrography am-ong others are able to separate and quantify isote-troin. However, the extraction is quite complex and in the case of HPLC, the analysis requires long reten-tion times. In such context, an isocratic reversed-phase high-performance liquid chromatography (HP-LC) technique coupled with an UV-vis detector i