Pages 1–11 Reverse Engineering of Gene Regulatory Networks for Incomplete Expression Data: Transcriptional Control of Haemopoietic

Motivation: The identification of the topology and function of gene regulation networks remains a challenge. A frequently used strategy is to reconstruct gene regulatory networks from time series of gene expression levels from data pooled from cell populations. However, this strategy causes problems if the gene expression in different cells of the population is not syn-chronous, as is expected to be the case in the transcription factor network that controls lineage commitment in haema-topoietic stem cells. Here, a promising alternative may be to measure the gene expression levels in single cells individually. The inference of a network requires knowledge of the gene expression levels at successive time points, at least before and after a network transition. However, due to experimental limitations a complete determination of the precursor state i