Developmental Cell 17 Supplemental Data Dual Modes of Cdc42 Recycling

(A) Representative images of budding cells expressing GFP-Cdc42 in the ∆rdi1 background, cells in the WT background after treatment with 100 µM LatA, and cells in the ∆rdi1 background treated with LatA. Scale bar is 2.0 µm. Removal of one of the two pathways does not result in loss of polarization, but removal of both pathways results in loss of polarity. (B) FRAP rates of Cdc42 in WT, Cdc42 in WT + LatA, Cdc42 in ∆rdi1, and the sum of all possible combinations of the rate of FRAP for Cdc42 in WT treated LatA, and Cdc42 in ∆rdi1. Box width is the standard error of the mean, whiskers represent the standard deviation. The rate of recovery of Cdc42 in WT is statistically indistinguishable from all combinations of summed rates of cells from the the individual pathways.