Multispectral fluorescence imaging

Multispectral fluorescence imaging (MSFI) is a rapidly growing field with broad applications in both preclinical and clinical set-tings. Application of this novel technology in small-animal imag-ing and microscopy produces enhanced sensitivity and reliable quantification and resolves multiple simultaneous signals. MSFI flow cytometry can quantify multiple fluorescent parameters with morphologic or subcellular spatial details on millions of cells. MSFI has the potential to improve the accuracy of dis-ease detection or differentiation and intrasurgical metastatic di-agnosis, guide neurosurgeries, andmonitor treatment response. Key Words: multispectral fluorescence imaging; multiplexing; spectral unmixing; small-animal imaging; microscopy; flow cy-tometr