Figure S1A-C. Generation of Tph2-deficient mice

Figure S1. (A) Targeting strategy for generating Tph2-/- mice through homologous recombination in embryonic stem (ES) cells. (B) β-galactosidase staining of different tissues of WT (left) and Tph2-/- (right) mice brain (a-e) [a: Cerebral cortex (dorsal view); b: Cerebral cortex (ventral view); c: Cerebellum; d and e: Brain stem]. Positive brain areas are highlighted with dotted yellow lines. (C) Schematic representation of locus of β-galactosidase-positive neurons (in blue) in adult mouse brain. DR: dorsal raphe; MR; median raphe; CR: caudal raphe Figure S1D. Characterization of Tph2 expression throughout the brain Figure S1D. Series of brain 20 µm cryosections (A-W) from Tph2/LacZ mice were stained with β-galactosidase at 37oC, counterstained in eosin, cleared and mounted in DPX. Pictures 1X and inset 5X. 2.46 mm 1.42 mm 0.26 mm-0.34 mm-1.22 mm-1.34 mm-1.58 mm-1.82 mm-1.94 mm-2.06 mm-3.28 mm-4.04 mm-4.24 mm-4.36 mm-4.60 mm-4.72 mm-4.84 mm-4.96 mm-5.02 mm-5.40 mm-6.00 mm-6.48 mm-7.32 mm Tph2-positive neurons are in blue. Please note that only brain regions with positive labeling have been shown in the 5X magnification. Bregma locations of sections are indicated below each panel. Figure S1E. Serum levels of T4 and Corticosterone in Tph2-/- mice Figure S1E. Serum T4 and corticosterone were measured by radio-immunoassay in Tph2-/- mice followin