Immunofixation. II: Application to Typing of a1-Antitrypsin at Acid pH

Technical and economic problems have slowed the in-troduction of genetic typing of a1-antitrypsin into the lab-oratory community. Immunofixation of the protein at acid pH makes protease inhibitor (Pi) typing feasible in any laboratory capable of performing standard protein elec-trophoresis in agarose. Additional Keyphrases: genetic typing #{149}plasma protein. electrophoresis #{149}pulmonary disease testing In 1964, Eriksson (1) discovered that the major plasma protease inhibitor (Pi), aj-antitrypsin (2),was under genetic control. Since the first observation of a deficiency of a1-anti-trypsin, in the serum of a Swedish patient (3), population studies have shown that there are at least 23 codominant al-leles that combine in classical mendelian fashion to effect differences in physicochemical properties and amount pro