and herpes simplex virus type 1 and identification of heparin-binding PRV glycoproteins

To determine whether heparan sulphate residues on the cellular surface could serve as an attachment receptor for pseudorabies virus (PRV), the effect of heparin on PRV in plaque reduction and adsorption tests was investigated. PRV was significantly less sensitive to heparin than was herpes implex virus type 1 (HSV-1). At concentrations of 500 lag/ml heparin the number of plaques formed by PRV was reduced to 7 % of the untreated control whereas the number of plaques formed by HSV-1 was reduced to below 0.1%. Adsorption of PRV to host cells was also less sensitive to heparin treatment than was adsorption of HSV-1. Experiments concerning the binding sites of PRV showed that heparin binds to the disulphide-linked glycoprotein complex glI (PRV gB), gill (PRV gC) and probably gV. The first events in the life cycte of.a herpesvirus-are attachment to the cellular surface and entry of the virus into the cytoplasm of its host cell. Many studies on the entry of herpesviruses into the cell have been don