Biophysical Journal, Volume 96 Supporting Material Novel Micropatterned Cardiac Cell Cultures with Realistic Ventricular Microstructure

Supplementary Figure S1. Optical mapping of electrical propagation in cardiac cultures. (A) Tyrode’s solution is gravity-perfused through a temperature-controlled chamber housing the coverslip with cells. Light signals from cells stained with potentiometric dyes are recorded by a 504-channel optical fiber array placed in contact with the bottom of the chamber, digitized, stored, and analyzed on a PC. Point “P ” stimuli are applied using Grass stimulator. (B) Top view of chamber showing position of cell culture slice and stimulus electrode relative to underlying optical fiber array. Note that the entire micropatterned cell culture fits inside the field-of-view of the recording array. (C) Example raw (black) and filtered (red) signals. Signal-to-noise ratio (SNR) is calculated as the ratio of the signal amplitude to the RMS of the baseline noise. Supplementary Figure S2. Continuity of pixel-to-pixel fiber transitioning. (A) Example F-actin-stained image illustrating dominant cell alignment (red line) in 160×160 μm2 pixel centers (dashed blue regions) and 15 μm wide pixel edges (dashed yellow regions). (B) The continuity of the fiber transitioning between neighboring pixels was quantified by comparing the change in fiber alignment between the edges of neighboring pixels (i.e., absolute fiber angle difference between adjacent yellow regions at either side of a border) to that between the correspondin