Ministry of Agriculture, Food Technology & Natural Resources

The infectious bursal disease virus was isolated in chicken embryofibroblast cell-culture (CEF) from bursal suspension of suspected dead chicks. The viral isolate was well adopted and titrated at sixteenth passage level in CEF. The serum neutralisation test in cell-culture and enzyme linked immunosorbant assay revealed that the isolate is of classical IBD serotype I. The morphology of IBD virus was also seen in electron microscopy of infected cell-suspensions. The details of the results are discussed