Bloodstream Form of Trypanosoma evansi Contains-Galactosidase

Abstract:-Galactosidase (EC 3.2.1.23) was isolated and characterized from the bloodstream form of Trypanosoma evansi. The galactose hydrolase was purified 6.98 fold with a yield of 33.33 % by anion exchange chromatography using DEAE- cellulose and size exclusion procedure on Sephadex G- 75 column. The T. evansi-Galactosidase had broad pH and temperature ranges with an optimum of 6.0 and 37°C respectively. Initial velocity studies for the determination of kinetic parameters using 4-methylumbelliferyl--D-galactoside (MU-Gal) as substrate revealed a K and V of 0.08mM and 0.07 µmol/min respectively. The enzyme activityM max was enhanced by cations: Fe, Co and Mg while Ca was found to inhibit it.2+ 2+ 2+ 2+ Key words:-Galactosidase Enzyme Galactosides T. evansi INTRODUCTION pathogenesis and pathophysiology of the T. evansi Trypanosoma evansi, the causative agent of surra, is the development of vaccine against trypanosomiasis. a heamoflagellate protozoan parasites that infects domestic animals causing animal trypanosomiasis. It has MATERIALS AND METHODS the broadest geographical and host range among th