The Ren1d cre Mouse, an Excellent Model to Defi ne the Renin Cell

Kidney section from a Ren1d-cre;R26R adult mouse subjected to the X-gal reaction and immunostained for α-SMA shows costaining of β-gal (blue) and α-SMA (brown) expression in the afferent arteriole (aa), in the interlobular artery (IA) connecting to the aa, and within the glomeruli (g) in the mesangial cells. Scale bar: 50 μ m. Figure S2. Renin-Expressing Cells Are Progenitors for Cells in Extrarenal Organs (A and B) Adrenal gland, (C–G) testis, (H and I) stellate ganglion. Adult Ren1d-cre;R26R (A–G) and newborn Ren1d-cre;Z/EG (H and I) mice. Counterstaining with eosin (A, B, and D). (A) Low magnifi cation of an adrenal gland showing the cortex (glomerulosa [g], fasciculata [f], and X-zone or reticularis [x/r] zones) and the medulla. β-gal expression (blue) occurs in a radially variegated pattern across all the cortical zones. (B) Higher magnifi cation of (A) showing cells from the renin lineage in all cortical layers. (C) Paraffi n section of the testis immunostained for cytochrome p450 side chain cleavage enzyme (scc) (brown) labels interstitial Leydig cells (arrows) between seminiferous tubules (st). Hematoxylin counterstaining. (D) Frozen section subjected to the X-gal reaction shows β-gal expression in interstitial Leydig cells (arrows). (E and F) Consecutive frozen sections show that the same cells that express β-gal (blue, [E]) correspond to Leydig cells that immunostain for cytochrome p450scc (brown, [F]). (G) Double labeling for β-gal and cytochrome p450scc shows coincidence of both markers in Leydig cells. We observed β-gal and GFP expres-sion in Leydig cells of fetal and newborn mice (not shown) indicating that renin is expressed during development of the testis