Measurement of total protein and albumin in serum with a centrifugal analyzer

We describe methods for measuring total protein and al-bumin with the centrifugal analyzer. The biuret reaction for total protein is done in the transfer disc, thus increasing analysis rate. Albumin is measured by the bromcresol green reaction. Precision of both methods is excellent and correlation studies demonstrate the validity of the proce-dures. In most clinical chemistry laboratories, mechanized pro-cedures are essential for measurement of serum total protein and albumin. The biuret reaction is well established as the most practical approach to mechanized total protein mea-surements (1-4); albumin has posed a more difficult problem, with several procedures being proposed, some of which have involved salt fractionation (1-4), electrophoresis (5), dye binding (6-9), or total globulin measurements (10, 11). Dy