中国农业科 学 2008,41(1):221-228

Abstract: 【Objective】The myostatin gene of Landes goose was amplified by reverse transcription-polymerase chain reaction (RT-PCR), and the relationship between MSTN gene expression and different energy diets, or IGF-I, GH levels in serum was researched in order to know the function of MSTN gene and provide information for molecular nutriology and breeding of water fowl. 【Method】Genome RNA was extracted from muscle of Landes goose, MSTN gene mRNA was amplified by RT-PCR, the PCR product was cloned into pGEM-T vector and transformed into Escherichia coli. The MSTN gene was isolated and sequenced from the positive clones screened. Based on the MSTN gene clone, β-actin as inner control, an optimal semi-quantitative RT-PCR method