Interleukin-11 Attenuates Ifosfamide-Induced Hemorrhagic

Objective: To investigate the possible protective effect of recombinant human interleukin-11 (rhIL-11) against ifosfamide (IFS)-induced hemorrhagic cystitis (HC). Materials and Methods: Male Swiss mice (20-30g) were pretreated with rhIL-11 (25-625 µg, subcutaneously.) 30 min before intraperitoneal injection of IFS (400 mg/kg) or with saline (control group). Twelve hours later, HC was evaluated by bladder wet weight (BWW) to quantify edema, Evans blue extravasation (EBE) to measure vascular permeability, and macroscopic and microscopic analysis. All bladders were assessed by histopathological analysis. Results: rhIL-11 (at 125 and 625 µg) attenuated the IFS- induced increase of BWW (37.48 % and 45.44%, respectively, p < 0.05) and EBE (62.35 % and 56.47%, respectively, p < 0.05). IFS- induced macroscopic edema and hemorrhage and micro-scopic alterations, were also prevented by rhIL-11 at 625 µg. (p < 0.05). Conclusion: Our results demonstrate a protective effect of rhIL-11 on experimental IFS- induced HC, not previously reported. Key words: bladder; ifosfamide; cystitis; interleukin-11; rat