Add to ORKGThe plasmids encoding myc-tagged Raptor, and HA-tagged S6K1 and RSK1-4 were previously described [1-3]. All Raptor point mutants were generated using the Quickchange methodology (Stratagene, La Jolla, CA). pGEX-Raptor was generated by inserting nucleotides 2095-2235 of human Raptor in pGEX-4T. The vectors encoding Flag-MEK1-DD, HA-ERK2, RasG12V, RasS17N and AU1-mTOR were previously described [1, 4]. The plasmids encoding Flag-mTOR and GST-4E-BP1 were kindly provided by Dr. Nahum Sonenberg (McGill University) and the plasmid encoding HA-ERK1 was provided by Dr. Sylvain Meloche (Université de Montréal)
The full coding sequences (without the termination codon) of smo and fu cDNAs were amplified by poly...
Yeast Mre11/Rad50: The S. cerevisiae Mre11 gene was amplified from a cDNA (Research Genetics) and cl...
Flag-Smad7 and HA-Smurf2 constructs were obtained from J. Wrana (U. of Toronto). Expression vectors ...
Several plasmids used in this study have previously been described. They include the expression plas...
The following plasmids were used for the generation of retroviruses: pBabe (Morgenstern and Land, 19...
(Langley et al., 2002). The plasmid encoding Myc-NBS1mt was generated using the QuickChange Site-Dir...
tained by PCR, sequenced, and cloned into pCMUIV vector [S1]. Dominant-negative Rab11A S25N and Rab4...
Plasmid pRJ741 (pG14-prGPD1-MS2CP-RedStar) was constructed by ligating pG14-GPD1-MS2CP-GFP [S1], whi...
pCMV-myc-avrA and pCMV-myc-mavrA are described previously (Collier-Hyams et al., 2002). The mAvrA mu...
described previously. The GST-Rho39 expression vector was constructed by transferring the sequence e...
Random mutagenesis of the plasmid pC901 encoding Lim-PKR (Ung et al., 2001) was performed using the ...
Standard molecular biology techniques were used for cloning (Sambrook, 2001). The structures of all ...
et al. 2007). pBabeSOCS1 and pBabeSOCS1ΔBox were obtained from Dr I. Touw. pLPCMyc-tagged SOCS1 was ...
The sequence of the λ5-VpreB1 locus is described in Minaee et al., 2005. Primer sequences for the λ5...
Spring Harbor, NY, 1993), vol. 2, pp. 843Ð896. 22. Rap1 mutant wing clones expressing a-cateninÐ GFP...
The full coding sequences (without the termination codon) of smo and fu cDNAs were amplified by poly...
Yeast Mre11/Rad50: The S. cerevisiae Mre11 gene was amplified from a cDNA (Research Genetics) and cl...
Flag-Smad7 and HA-Smurf2 constructs were obtained from J. Wrana (U. of Toronto). Expression vectors ...
Several plasmids used in this study have previously been described. They include the expression plas...
The following plasmids were used for the generation of retroviruses: pBabe (Morgenstern and Land, 19...
(Langley et al., 2002). The plasmid encoding Myc-NBS1mt was generated using the QuickChange Site-Dir...
tained by PCR, sequenced, and cloned into pCMUIV vector [S1]. Dominant-negative Rab11A S25N and Rab4...
Plasmid pRJ741 (pG14-prGPD1-MS2CP-RedStar) was constructed by ligating pG14-GPD1-MS2CP-GFP [S1], whi...
pCMV-myc-avrA and pCMV-myc-mavrA are described previously (Collier-Hyams et al., 2002). The mAvrA mu...
described previously. The GST-Rho39 expression vector was constructed by transferring the sequence e...
Random mutagenesis of the plasmid pC901 encoding Lim-PKR (Ung et al., 2001) was performed using the ...
Standard molecular biology techniques were used for cloning (Sambrook, 2001). The structures of all ...
et al. 2007). pBabeSOCS1 and pBabeSOCS1ΔBox were obtained from Dr I. Touw. pLPCMyc-tagged SOCS1 was ...
The sequence of the λ5-VpreB1 locus is described in Minaee et al., 2005. Primer sequences for the λ5...
Spring Harbor, NY, 1993), vol. 2, pp. 843Ð896. 22. Rap1 mutant wing clones expressing a-cateninÐ GFP...
The full coding sequences (without the termination codon) of smo and fu cDNAs were amplified by poly...
Yeast Mre11/Rad50: The S. cerevisiae Mre11 gene was amplified from a cDNA (Research Genetics) and cl...
Flag-Smad7 and HA-Smurf2 constructs were obtained from J. Wrana (U. of Toronto). Expression vectors ...