Biochemistry Lebanese University

Abstract:-E. coli strain TB1 are used to determine the effects of microwaves (MW) of frequency 900 MHz and an intensity of field exposure 6 V/m at ambient temperature. Our experiment was preformed by studying the effect of MW on DNA. We transformed unexposed bacteria with exposed pUC18 plasmid. We did not find any variation in the transformation ratio (100 transformants/μg DNA). We did not find any variation in the number of blue colonies (100 % blue colonies). Analysis of exposed DNA with quantitative PCR technique was realized to determine the quantity of broken DNA strands after MW exposure. By comparison between exposed and control DNA no difference was observed. Electrophoresis and spectroscopic analysis of exposed DNA did not reveal any hyperchrome effect. In order to confirm our results we sequenced exposed pUC18 plasmid but again no alteration of the DNA on the molecular level was observed