In vitro Regeneration of Garlic Through Callus Culture

Abstract: Garlic (Allium sativum) root tips were induced to regenerate shoots through callus culture and somatic embryogenesis to obtain plantlets. The experiment was designed to investigate the regeneration potentiality of two garlic verities and also to develop an efficient protocol for regeneration of garlic via callus culture. Higher percentage of callus was initiated from the combination of 5 mg lG Kinetin and 1.5 mg lG1 1 2,4-D. Embryogenic callus produced higher number of shoots in MS medium supplemented with 10 BAP. Rooting of individual shoots was induced after transfer to medium without growth regulator. The plantlets were established in the soil after acclimization. Cultivar differences in regeneration from root tips were observed. Key words: In vitro, garlic, callus INTRODUCTION solution with 2 drops Tween–20 per 100 ml for 5 minutes Garlic (Allium sativum L.) is an important and widely Root tips of spouted cloves about 2-3 mm long were used cultivated plant with both culinary and medical uses as explants. Explants were placed on MS medium stemming from its biological activities, which include supplemented with different concentrations (0, 0.5, 1.0, antibiotic, anticancer, antithrombotic and lipid lowering 1.5, 2. mg lG) of 2.4-D with the combination of BAP (0, 5