Sequence analysis and product assignment of segment 7 of the rice dwarf virus genome

The complete nucleotide sequence of segment 7 of the rice dwarf virus (RDV) genome was determined. The segment was 1696 bp long and its plus-strand terminal sequence, 5'GGCAAA---UGAU 3', was in agreement with the consensus sequence previously found in other segments of RDV. A 10 bp inverted repeat was found adjacent o the termini. A single long open reading frame extended for 1518 bp from the first AUG triplet (positions 26 to 28), and encoded apolypeptide of 506 amino acids (Mr 55 339). This protein had 32 % identity in the amino acid sequence to the 57K protein encoded by segment 7 of the wound tumour virus genome. The translation product of transcript RNA made from 'tailored ' cDNA of RDV segment 7 comigrated with the 60K core protein of RDV in 10 % polyacrylamid