Benchmarks

Proximity ligation assay (PLA) is a novel protein detection strategy based on the transformation of detected proteins into DNA sequences through a DNA ligation reaction (1–6). This conversion of protein into DNA strands is followed by DNA amplification and detection, for example, by quantitative PCR or rolling circle amplification (RCA). One further development of the basic proximity ligation strategy is the triple-specific proximity ligation assay (3PLA), in which three independent recognition events are required to give rise to a specifi