The use of high pressure liquid chromatography to investigate the in vitro reactions of human erythrocytes with guanosine. Anal. Chem

The development of high-pressure liquid chromatog-raphy has made available a new technique for moni-toring nucleotide concentrations in biological materi-als sensitively, accurately, and rapidly. Because there appears to be a correlation between ATP con-centrations and viability of stored blood, a prelimi-nary study was carried out on blood cell suspensions to evaluate the technique for possible use in blood banks, clinical laboratories, and research on preser-vatives for blood. Additlonal Keyphrases: viability of stored blood blood banking #{149}ATP, ADP, AMP #{149}blood preserva-tion The recent development of high-pressure liquid chromatography (HPLC) has introduced a new tech-nique for monitoring nucleotide concentrations in bi-ological materials (1, 2). Other methods are available for the determination of ATP (3) and other nucleo-tides; however, an advantage in using HPLC is that the major nucleotides can be determined at one time. In addition, the analyses are accomplished with speed, sensitivity, accuracy, and ease of opera-tion. A decrease in the concentrations of organic phos-phate, especially that of ATP, is closely associated with the loss of viability of stored blood (4-7). The relationship between viability and ATP was investi-gated by Gabrio et al. (8, 9) and they found that the post-transfusion survival time of stored blood was substantially increased when the ATP concentra-tions were elevated through incubation with adeno-sine. Because there appears to be a correlation be