Principles and Applications of High-Performance Ion-Exchange Chromatography for Bioseparations Introducing Vydac VHP High Performance Ion-Exchange Columns VHP High-Performance Ion-Exchange Columns Principles of High-Performance Ion-Exchange Chromatography

Ion-exchange chromatography separates proteins by charge primarily through electrostatic interactions between charged amino acid side chains and the surface charge of the ion-exchange resin. Protein retention has been explained as a "net charge " phenomenon in which a protein is considered to be a point charge and retention is a function of the net charge of the protein at the pH of the mobile phase. Kopaciewicz and col-leagues, however, have developed a more comprehensive mechanism of the ion-exchange chromatography o