Statistical Optimization of Cyclosporin A Production on a Semi-Synthetic Medium Using Tolypocladium inflatum MTCC 557

Abstract: Statistical technique was used to optimize the fermentation conditions for maximum production of cyclosporin A (CyA) using T. inflatum MTCC 557. Various carbon and nitrogen sources were screened for the maximum production of CyA. A response surface methodology (RSM) involving 4 factors and 5 levels was adopted to acquire the best medium for the production of CyA. Thus polynomial model was created to correlate the relationship between four variables and CyA yield. The optimal combination of media components included (in g/l) glucose 58.46 casein peptone 8.66, KH PO 4.48 and KCl 3.23 that gave maximum production of 134.5 mg/l2 4 CyA which was 2.7-fold more than the basal media. Subsequently, the effect of inoculum age, inoculum size and amino acids on the production of CyA was studied. Key words: Submerged fermentation Cyclosporin A Response surface method Tolypocladium inflatum INTRODUCTION the fermentation where the composition and titer of each Cyclosporin A (CyA), a cyclic undecapeptide, is one externally supplemented amino acids. of the most commonly prescribed immunosuppressive The effect of different media components as carbon drugs for the treatment of patients with organ and nitrogen sources [6, 12] and environmental factors transplantation, autoimmune diseases, including AIDS, such as pH and inoculum density [13] on CyA productio