The redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) was employed for direct epifluorescent microscopic enumeration of respiring bacteria in environmental samples. Oxidized CTC is nearly colorless and is nonfluorescent; however, the compound is readily reduced via electron transport activity to fluorescent, insoluble CTC-formazan, which accumulates intracellularly. Bacteria containing CTC-formazan were visual-ized by epifluorescence microscopy in wet-mount preparations, on polycarbonate membrane filter surfaces, or in biofilms associated with optically opaque surfaces. Counterstaining of CTC-treated samples with the DNA-specific fluorochrome 4',6-diamidino-2-phenylindole allowed enumeration of active and total bacterial subpopu...
Direct Fluorescent Antibodies (DFA) and Most Probable Number (MPN) techniques are currently being em...
Abstract Increasing evidence suggests that bacteria are capable of creating specific redox condition...
During the last 10 years, the dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) has been used to de...
Article appears in Aquatic Microbial Ecology and is copyrighted by Inter Research.The redox dye 5-cy...
Direct counting techniques, first developed for aquatic samples, can be used to enumerate bacteria i...
Tap water collected according to standard methods was examined for microbial presence. Epifluorescen...
Flow cytometry was used to study starvation of Escherichia coli in artificial seawater using two mem...
Bacteria play a fundamental role in the cycling of nutrients in aquatic environments. A precise dist...
The tetrazolium salt sodium 3\u27-{1-[(phenylamino)-carbonyl]-3,4- tetrazolium}-bis (4-methoxy-6-nit...
Le système transporteur d'électron des bactéries réduit le 2-(p-iodophenyl) -3(p-nitrophenyl) -5-phe...
A novel fluorescence technique for monitoring the redox status of c-type cytochromes in Geobacter su...
A colorimetric method for quantitative measurement of the oxidation/reduction potential (ORP) in liq...
Increasing evidence suggests that bacteria are capable of creating specific redox conditions which a...
Aims To develop a staining method for specific detection of metabolically active (viable) cells in b...
ABSTRACT A simple test for toxic chemicals has been developed. Rhizobium meliloti is combined with t...
Direct Fluorescent Antibodies (DFA) and Most Probable Number (MPN) techniques are currently being em...
Abstract Increasing evidence suggests that bacteria are capable of creating specific redox condition...
During the last 10 years, the dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) has been used to de...
Article appears in Aquatic Microbial Ecology and is copyrighted by Inter Research.The redox dye 5-cy...
Direct counting techniques, first developed for aquatic samples, can be used to enumerate bacteria i...
Tap water collected according to standard methods was examined for microbial presence. Epifluorescen...
Flow cytometry was used to study starvation of Escherichia coli in artificial seawater using two mem...
Bacteria play a fundamental role in the cycling of nutrients in aquatic environments. A precise dist...
The tetrazolium salt sodium 3\u27-{1-[(phenylamino)-carbonyl]-3,4- tetrazolium}-bis (4-methoxy-6-nit...
Le système transporteur d'électron des bactéries réduit le 2-(p-iodophenyl) -3(p-nitrophenyl) -5-phe...
A novel fluorescence technique for monitoring the redox status of c-type cytochromes in Geobacter su...
A colorimetric method for quantitative measurement of the oxidation/reduction potential (ORP) in liq...
Increasing evidence suggests that bacteria are capable of creating specific redox conditions which a...
Aims To develop a staining method for specific detection of metabolically active (viable) cells in b...
ABSTRACT A simple test for toxic chemicals has been developed. Rhizobium meliloti is combined with t...
Direct Fluorescent Antibodies (DFA) and Most Probable Number (MPN) techniques are currently being em...
Abstract Increasing evidence suggests that bacteria are capable of creating specific redox condition...
During the last 10 years, the dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) has been used to de...