PM 701 a Highly Selective Anti Cancerous Agent Against L1210 Leukemic Cells: In Vivo Clinical and Histopathological Study

Abstract. Cancer therapy remains a challenging problem. PM 701 is an ideal anti-cancer agent, which selectively targets cancer cells, thereby sparing normal tissues. In the first stage of this study, PM 701 induced programmed cell death (apoptosis) on cancer cells without any side effects on normal cells at tissue culture level. The aim of this study was to test the effect of PM 701 agent on cancer-induced animal models which were successfully produced at our laboratory. L1210 leukemia cells were inoculated intraperitoneally in adult male MFI mice which were divided into 4 groups. Group One – injected intraperitoneally with leukemia cells without treatment; Group Two – injected with L1210 cells and treated with oral administration of (PM 701) at the end of the 1st week; Group Three – received PM 701 only, and Group Four – control mice that drank only distilled water. The animal model was successfully produced when the inoculated tumor cells were grown locally and produced distant metastases. This was confirmed by the growth of peritoneal mass, positive blood smear and histopathological study which revealed systemic infiltration of leukemia cells in the liver, lung, kidney, spleen and testes. The use of PM 701 in cancer induced animals indicated its efficacy which showed marked improvement of the animals ' clinical condition, improved animal survival rate, negative blood smear; decrease in the size of pertioneal mass, reduction of metastatic deposits in some organs and eradication in others