Supplemental Data S1 Drosophila Stathmin Is Required to Maintain Tubulin Pools

Flies were raised on standard medium at 25C. stathmin is an essen-tial gene in Drosophila, and the stathminL27 mutant is described in [S1]. It is a small deletion that completely removes the single Drosophila stathmin gene. Expression of an adjacent gene also removed by the deletion and expected to encode a component of the Arp2/3 actin-regulatory complex (arc-p20) was rescued by a transgene in all experiments. Without the arc-p20 rescue con-struct, additional actin-related phenotypes were observed: small ring canals and dumping defects, as seen for other Arp2/3 mutants [S2]. The stathmin rescue construct used was tub-stathminA. Clones were induced by heat shocking larvae of the genotype hsFLP/+; FRT40, stathminL27/FRT40, Ubi-GFP; tub::arc-p20/+ or hsFLP/+; FRT40, stathminL27/FRT40, ovoD; tub::arc-p20/+ as indicated. For adult heat shock, females were heat shocked 3 days before analysis