Overexpressing a Truncated Form of EMSY

The EMSY gene encodes a protein that interacts with Brca2 and is amplifi ed in some sporadic cases of human breast cancer. To examine whether overex-pression of EMSY would mimic the chromosome instability phenotype that is associated with the loss of Brca2 function, we constructed a lentiviral vector (Lenti-EMSY/GFP) that en-codes a truncated form of the Emsy protein, including its Brca2-interacting domain, and green fl uorescent protein (GFP) and used it to transduce hu-man telomerase- immortalized human breast epithelial (184-hTert) cells